Analysis of Arbovirus Ribonucleic Acid Forms by Polyacrylamide Gel Electrophoresis
نویسندگان
چکیده
منابع مشابه
Analysis of arbovirus ribonucleic acid forms by polyacrylamide gel electrophoresis.
Viral ribonucleic acid (RNA) from Semliki Forest virus- and Sindbis virus-infected cells was analyzed by electrophoresis on polyacrylamide gels. In contrast to earlier results obtained by sucrose density gradient centrifugation, all of the known viral RNA forms (i.e., the 42S, 26S, replicative form, and replicative intermediate) were very clearly separated. The high resolution of the electropho...
متن کاملIdentification of L Forms by Polyacrylamide - Gel Electrophoresis THEODORE
Crude membranes were obtained from L forms by allowing the cells to lyse in distilled water. The crude membranes were washed several times in distilled water, lyophilized, and extracted with phenol-acetic acid-water. The membrane proteins were separated electrophoretically in polyacrylamide gels run at pH 4.5. Electrophoretic patterns and densitometric tracings of the gels showed distinct, repr...
متن کاملIdentification of L forms by polyacrylamide-gel electrophoresis.
Crude membranes were obtained from L forms by allowing the cells to lyse in distilled water. The crude membranes were washed several times in distilled water, lyophilized, and extracted with phenol-acetic acid-water. The membrane proteins were separated electrophoretically in polyacrylamide gels run at pH 4.5. Electrophoretic patterns and densitometric tracings of the gels showed distinct, repr...
متن کاملMolecular weight of Sindbis virus ribonucleic acid as measured by polyacrylamide gel electrophoresis.
The molecular weight (MW) of Sindbis virion ribonucleic acid (RNA) determined by gel electrophoresis was in a range of 3.89 x 10(6) to 4.45 x 10(6) daltons. Upon denaturation with urea, it separated into fragments having an MW of 1.76 x 10(6) daltons. Viral-specific Sindbis 26S RNA contained a major species having an MW of 1.76 x 10(6) daltons.
متن کاملSeparation of bacterial ribosomal ribonucleic acid from its macromolecular precursors by polyacrylamide gel electrophoresis.
Electrophoresis on polyacrylamide gels was found to be a powerful technique for separating the mature from the precursor forms of bacterial ribosomal nucleic acid (rRNA). The separation of the 16S rRNA from its precursor was, for all practical purposes, complete; that of the 23S rRNA from its precursor was detectable but incomplete. When mature and precursor rRNA preparations were heated to ran...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1971
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.7.4.504-514.1971